HEPATOTOXICITY OF THE ETHANOLIC EXTRACTS OF Enantia chlorantha ROOT ON WISTAR ALBINO RATS

ABSTRACT

The root of Enantia chlorontha was assayed for its hepatotoxicity effect. Extacts from the root were made using 95% ethanol. The root extracts were administered orally to 15 wistar albino rats and varying doses. 5 rats were kept as control group. The effect of the extracts was determined by assaying for the levels of serum-liver enzymes acting as biochemical makers. Liver enzymes assayed included Aspartate aminotransferase (AST), Alanin aminiotransferase (ALT) and alkaline phophatase (ALP). Comparison of the enzyme levels of the control group with these of the group administered with the rot extract revealed a significant elevation of liver enzyme levels (P

Table of Contents:

TABLE OF CONTENTS

TITLE PAGE ICERTIFICATION IIDEDICATION IIIACKNOWLEDGEMENT IVTABLE OF CONTENT V

ABSTRACT IX

CHAPTER ONE1.0 INTRODUCTION 11.1 BACKGROUND OF THE STUDY 11.2 AIMS AND OBJECTIVES 61.3 STATEMENT OF PROBLEM 71.4 LIMITATION OF STUDY 7

1.5 SIGNIFICANCE OF THE STUDY 8

CHAPTER TWO2.0 LITERATURE REVIEW 92.1 GENERAL DESCRIPTION OF Enantia chlorontha 102.2 ECOLOGICAL DISTRIBUTION OFEnantia chlorantha 102.3 MANAGEMENT AND PROPAGATION PRACTICES 112.4 FUNCTIONAL USES 122.5 TOXICITY 132.6 HEPATOTOXICITY 172.7 LIVER 202.7.1 LIVER ENZYME 222.7.2 SOME PATTERN OF LIVER INJURY 232.7.3 TREATMENT/PROGNOSIS OF LIVER DAMAGE 282.8 THE ALBINO RATS 29

2.9 PHYTOCHEMICAL COMPOUNDS 31

CHAPTER THREE3.0 MATERIALS AND METHODS 323.1 COLLECTION, IDENTIFICATION ANDPROCESSING OF PLANT MATERIAL 323.1.1 EXTRACTION OF PLANT MATERIAL 333.1.2 DETERMINATION OF THE WEIGHT OFDRIED EXTRACT 343.2 PHYTOCHEMCIAL ANALYSIS 353.3 EXPERIMENTAL ANIMAL 433.4 DETERMINATION OF DOSAGE OF THE PLANTEXTRACT FOR ANIMAL FEEDING 433.5 GROUPING OF ANIMAL AND ADMINISTRATIONOF PLANT EXTRACT 443.6 FEEDING METHOD 453.7 PROCEDURES FOR BLOOD SAMPLE COLLECTION 463.7.1 ALANINE AMINOTRANSFERASE TEST (ALT)METHOD FOR ALT TEST 473.7.2 ASPARTATE ANIMOTRANSFERASE: (AST)METHOD FOR AST TEST 483.7.3 ALKALINE PHOUSPHATASE: (ALP)METHOD FOR ALP TEST 49CHAPTER FOUR4.0 RESULTS 504.1 RESULT OF THE PHOTOCHEMICALANALYSIS OF THE ETHANOLIC EXTRACTOF Enantia chlorontha 50

4.2 HEPATOTOXICITY ASSAY 55

CHAPTER FIVE5.1 DISCUSSION 585.2 CONCLUSION 60

5.3 RECOMMENDATION 60

REFERENCES 61.

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