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ABSTRACT
This research work was carried out to determine the effect of Menzoneuron benthamainum on fasting blood sugar level of adult wistar rats. Twenty wistar rats (both male and female) weighing between 140g to 160g were used for this research project. They were acclimatized for 14 days after which the animals were grouped into four groups of five rats each. Each animal were administered based on their body weight. The dose given to animals in group A was 300mg/kg body weight of Menzoneuron benthamainum, Group B was given 500mg/kg body weight of Menzoneuron benthamainum, Group C was given 700mg/kg body weight of Menzoneuron benthamainum and group D (control group) was given distilled water. The administration lasted for thirteen days. Blood samples were collected from the tail of the animals and the Lipid profile level for each of the animals was tested.
TABLE OF CONTENTS
Contents
Title page
Certification
Dedication
Acknowledgements
Table of contents
List of Figures
List of Table
Abstract
CHAPTER ONE
1.0 Introduction
1.1 Medicinal Plants
1.2 Aim of Study
CHAPTER TWO
2.0 Literature review
2.1 Medicinal Plants and their uses
2.1.1 Mezoneuron benthamianum baill
2.2 Lipid Profile
2.2.1 Effect of medicinal plants on Lipid profile
CHAPTER THREE
3.0 Materials and Methods
3.1 Apparatus and Equipments
3.2 Chemical Reagents
3.3 Collection and extraction of plant materials
3.3.1 Mezoneuron benthamianum baill
3.4 Practical history and animal management
3.5 Drug administration
3.6 Sacrificing of Rats
3.7 Biochemical Analysis
3.7.1 Triglycerides
3.7.1.1 Principle
3.7.1.2 Reagent composition
3.7.1.3 Preparation of reagents
3.7.1.4 Procedure
3.7.2 HDL- Cholesterol
3.7.2.1 Principle
3.7.2.2 Preparation of reagents
3.7.2.3 Procedure
3.8 Statistical Analysis
CHAPTER FOUR
4.0 Results
CHAPTER FIVE
5.0 Discussion, Conclusion and Recommendation
5.1 Discussion
5.2 Conclusion
5.3 Recommendation
References
LIST OF FIGURES
Figure 1 –Mezoneuron benthamianum baill
Figure 2 – Bar chart showing the level of cholesterol in adult wistar rats
Figure 3 – Bar chart showing the level of HDL in adult wistar rats
Figure 4 – Bar chart showing the level of LDL in adult wistar rats
Figure 5 – Bar chart showing the level of triglycerides in adult wistar rats
LIST OF TABLE
Table 1 – Groups and treatment administered
Table 2- Procedure triglyceride determination
Table 3 – Procedure for HDL determination
Table 4- Cholesterol CHOD-PAP assay
Table 5- Showing the level of cholesterol, HDL, LDL and
triglycerides in adult wistar rats.
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